Vet Info-H • Sep–Dec 2023 • Vol 11 • No. 4 5 A indica is an ingredient of • Erina® (Coat Cleanser) • Erina®-EP (Dusting Powder, Shampoo) • Erina® Plus (Coat Cleanser With Conditioner) • Scavon® VET (Cream, Spray) • Liv.52® PROTEC (Liquid, Powder) • Geriforte® VET (Liquid, Powder) Antifungal Property A study was conducted to evaluate the antifungal activity of A indica leaves against the fungus strains— Aspergillus flavus, Alternaria solani, and Cladosporium species. Methanolic and ethanolic (25%, 50%, 75%, and 100%) extracts of the dried leaves of A indica were prepared. The inoculums of the fungal strains were prepared in sabouraud dextrose agar for the disc diffusion assay. Discs loaded with the methanolic and ethanolic extracts of A indica leaves were placed in the petri plates containing the inoculums. The antifungal activity was assessed by measuring the zone of inhibition.4 Results showed inhibition of fungal growth with both methanolic and ethanolic extracts of A indica in a dosedependent manner. Effective inhibition was noticed with the methanolic extract of A indica against A flavus. Thus, the study demonstrated the antifungal activity of A indica leaves.4 Parasicitidal Property A recent study evaluated the parasiticidal activity of A indica leaves against the parasites—Eimeria papillata and Allolobophora caliginosa. Methanolic extract of A indica leaves was prepared using 70% methanol. The anthelmintic property was assessed using 200, 100, and 50 mg/mL of methanolic A indica leaf extract against A caliginosa (5 worms/dose). After paralysis and death of the worms, histological examination was performed. The anticoccidial property was assessed with laboratory mice inoculated with 1 × 105 sporulated oocysts of coccidial murine parasite. Feces samples were collected on day 5 post infection, and oocysts were separated using flotation technique. The unsporulated oocysts (1 × 105) were treated with the methanolic A indica leaf extracts (100, 50, and 25 mg/mL). Sporulation and inhibition percentage of oocysts were evaluated.5 A dose-dependent efficacy of methanolic A indica leaf extract was observed in both the studies. Thus, the study confirmed significant parasiticidal activity of A indica leaves.5 Scientific Evidence Traditional Uses of A indica6 1. The green twigs of A indica were used as toothbrushes for cleaning teeth. 2. The bark, gum, leaf, and seeds of A indica were used to treat snake bites and scorpion stings. 3. The decoction of the leaves of A indica was used to treat ulcers and eczema. 4. A indica seed oil was used to treat rheumatism. References 1. Mehta N, et al. Int J Curr Microbiol App Sci. 2021;10(2):3228–3235. 2. Alzohairy MA. Evid Based Complement Altern Med. 2016;2016:7382506. 3. Asif M. J Pharmacogn Phytochem. 2012;1(4):78–83. 4. Shrivastava DK, Swarnkar K. Int J Curr Microbiol App Sci. 2014;3(5):305–308. 5. Hawsah MA, et al. Food Sci Technol. 2023;43(2):e117122. 6. Reddy IVS, Neelima P. Int J Econ Plants. 2022;9(1):59–63.
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