Vet Info-H • May–Aug 2023 • Vol 11 • No. 3 5 T cordifolia is an ingredient of • Anxocare® VET (Tablets) • Appetonic® VET (Bolus, Powder) • Diarex® VET (Bolus) • Digyton® (Drops) • Geriforte® VET (Liquid, Powder) • Himlay® (Powder) • Himpyrin® (Liquid) • Immunol® (Liquid) Antioxidant and Antiproliferative Properties A study was conducted to evaluate the free radical scavenging and antiproliferative abilities of T cordifolia stem. The ethanolic extract of T cordifolia stem and its subsequent petroleum ether, dichloromethane, n-butanol, and aqueous fractions were prepared. The antioxidant activity of the ethanolic extract and the 4 fractions were evaluated using 2,2-diphenyl-1-picrylhydrazyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical, and nitric oxide scavenging activity, iron chelating activity, and total antioxidant capacity assays. The antiproliferative ability of the ethanolic extract and the 4 fractions was evaluated through 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide and sulforhodamine B assays using human cervical cancer cell (HeLa) cultures.2 The results revealed a significant, dose-dependent radical scavenging activity and a dose-dependent inhibition of cell proliferation in HeLa cell line by the ethanolic extract and the 4 fractions. Further, n-butanol fraction exhibited superior free radical scavenging activity, and dichloromethane fraction exhibited the most significant antiproliferative activity. Thus, it can be concluded that T cordifolia stem possesses potent antioxidant and antiproliferative properties.2 Anxiolytic Property A study demonstrated the anxiolytic property of T cordifolia leaves. Three albino mice experimental groups were orally treated with the ethanolic extract of T cordifolia leaves at doses 100, 200, and 400 mg/kg body weight, respectively, for 7 consecutive days. The other 2 groups (control and standard groups) were treated with 0.6% w/v carboxymethyl Scientific Evidence cellulose and 1 mg/kg diazepam, respectively, for the same duration. On day 8, 1 hour after repeating the oral administration of the standard and ethanolic extracts, elevated plus maze test, light and dark transition test, and open field model test were performed for evaluating exploratory behavior, anxiety characteristics, and motor activity in all the mice groups.4 The results confirmed that the mice treated with the ethanolic leaf extract of T cordifolia produced an anxiolytic effect in a dose-dependent manner, and significant anxiolytic activity was found at 400 mg/kg body weight. Thus, it can be inferred that T cordifolia leaves possess potent anxiolytic property.4 References 1. Wasate P, et al. World J Pharm Life Sci. 2021;7(12):28–36. 2. Polu PR, et al. BMC Complement Altern Med. 2017;17:457. 3. Sinha K, et al. Indian J Tradit Knowl. 2004;3(3):257–270. 4. Gururaja MP, et al. J Young Pharm. 2022;14(1):46–49.
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