8 • Probe •Vol LXII • No. 3 • May–Aug 2023 Immunotherapeutic Modification by Septilin intravenously administered 0.1 mL of colloidal carbon after a warm-up period of 15 to 20 min at 37°C. One drop of blood was collected at 2, 5, 10, 15, 20, 20, 30, 45, 60, and 90 min by tail nipping. The graph of absorbance against time was plotted, and the area under curve determined. Humoral response in normal and immune-deficient rats In this method, wistar rats (n = 106) were divided into 4 groups: group 1 rats (n = 35) received water (10 mL/kg body weight), once daily, orally from day (- 9) to day (+ 4). Group 2 (n = 26) rats received 10 mL/kg body weight of water, once daily, orally from day (- 9) to day (+ 4), and on day (+ 2), 400 mg/kg body weight of cyclophosphamide was administered orally in addition to water treatment. Group 3 rats (n = 36) received Septilin syrup at a dose of 3 g/kg body weight, once daily, orally from day (- 9) to day (+ 4), and on day (+ 2), 400 mg/kg body weight of cyclophosphamide was administered orally in addition to Septilin treatment. All parameters were statistically analyzed either by the unpaired Student’s t test or the c2 test. Results Effect of Septilin on E coli–induced abdominal sepsis There was no mortality in the group treated with Septilin compared with 77.8% mortality in the control group 24 hours after receiving E coli cells (Figure 1). After 15 days of treatment with Septilin, total leukocyte, absolute lymphocyte, and absolute neutrophil counts significantly increased compared with that in the control group (Figure 2). Effect of Septilin on bactericidal activity of polymorphonuclear cells The total number of viable E coli cells (× 106/mL) in the Septilin-treated group (1.36 ± 0.39) was significantly lower compared with that in the control group (3.50 ± 0.77). The absolute neutrophil count (× 103/mL) in the Septilin-treated group (1786.00 ± 163.66) was significantly more than that in the control group (1243.50 ± 155.53). Figure 1. Effect of Septilin on Mortality in Escherichia coli– Induced Abdominal Sepsis Figure 2. Effect of Septilin Treatment on Leukocyte Counts a,b,cP < .001. Continued on page 12… 20 40 60 24 h 48 h 80 No mortality in the Septilin Rx group Control Septilin 100 0 Mortality, % 3000 2250 1500 750 a b a b c c 3750 4500 Total WBC Absolute Neutrophil Absolute Lymphocyte 5250 6000 Control (n = 15) Septilin (n = 15) 6750 0 Count (x 103/mL)
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