Probe • Vol LXII • No. 3 • May–Aug 2023 • 7 Preclinical Evidence Septilin® (SYRUP, TABLET) Septilin is recommended for the treatment and management of various infections and to prevent their recurrence. Septilin augments host response, reduces inflammation, and corrects the underlying pathologic features associated with recurrent infections. When co-prescribed with antibiotics, Septilin ensures faster recovery and prevents recurrence. Septilin is a valuable adjuvant in infection management and provides excellent short-term and long-term safety. Immunotherapeutic Modification by Septilin Aim To evaluate the effect of Septilin on both nonspecific and specific defense mechanisms in experimental rat and mice models Materials and Methods The study included Swiss albino mice and wistar rats for different study methods. Escherichia coli–induced abdominal sepsis in mice This method was carried out in 2 parts. The first part involved subculturing the pathogenic strain of E coli in the nutrient broth at 37°C and 50 rpm for 18 to 24 hours. The vitality of culture was determined using repeated dilution and surface spreading methods. The total viable cell count on the MacConkey agar plates was determined after a 24-hour incubation period. The ability of E coli to cause 100% mortality for up to 48 hours was tested. In the second part, 33 Swiss albino mice (weighing between 25 and 35 g) were divided into 2 groups: group 1 (control, n = 18) received tap water, and group 2 (n = 15) received Septilin syrup at a dose of 20 mL/kg body weight. Blood was collected for total and differential leukocyte counts. E coli (5 × 108) cells were injected intraperitoneally into mice in both the groups, and percentage mortality was observed after 24 hours. Bactericidal activity of polymorphonuclear cells in rats This was an in vitro method, in which the subcultured E coli was washed 3 times, a known volume of phosphate buffered saline was suspended, and the turbidity of the suspension was measured spectrophotometrically at 540 nm. The required viable E coli cells (1.6 × 107) were determined. A total of 28 wistar rats were divided into 2 groups: group 1 (control, n = 16) received water at a dose of 10 mL/kg body weight, and group 2 (n = 12) received Septilin syrup at a dose of 10 mL/kg body weight orally, once daily, for 15 days. Venous blood (8 mL) was collected. Staphylococcus aureus–induced sepsis in neutropenic mice This method included 27 albino mice and were randomized into 2 groups: group 1 (n = 15) received water at a dose of 20 mL/kg body weight, and group 2 (n = 12) received Septilin syrup at a dose of 20 mL/kg body weight, once daily, orally for 15 days. Carbon clearance assay in mice Swiss albino mice (n = 15) were divided into 2 groups: group 1 (n = 9) received 10 mL/kg body weight of tap water, and group 2 (n = 6) received 10 mL/kg body weight of Septilin syrup, orally, once daily, for 15 days. On day 15, both the groups were
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